P-305 Oxidized-LDL promotes colorectal cancer progression and growth of human colonoides

Expression levels of the oxidized-LDL receptor LOX-1 have been related to cancer progression in prostatic, gastric, pancreatic, and colorectal (CRC); however, relationship among pro-inflammatory markers, ox-LDL/LOX-1 CRC are not completely elucidated. We tested hypothesis that stimulation cell lines with TNF-α ox-LDL promotes tumor progression. also explore effect on human colonoids growth. The (CRC) HCT116 COLO320 were treated for expression. proliferation this proinflammatory signals assayed by MTT count exclusion dye trypan blue. LOX-1-negative obtained transduction lentiviral vectors encoding a shRNA specific LOX-1. expression genes involved analyzed qRT-PCR Western blot wild type negative cells ox-LDL. Cell migration invasion was cells. Human stimulated presence NADPH oxidase inhibitors ML171, VAS2870 ROS scavenger NAC. Surface area survival assessed contrast microscopy MTT. Proliferation click-chemistry. is expressed COLO320. Additionally, we found microarrays data stored public databases mRNA over-expressed carcinomas adenocarcinomas compared normal colon tissue. Treatment augmented silencing abrogated effect. Moreover, both proliferated dose-dependent manner. Importantly, organoids viability manner, an partly mediated ROS. activation induced angiogenic markers such as MMP2, MMP7, MMP9 VEGF. oxLDL epithelial mesenchymal transition cells, characterized reduced marker E-cadherin increased Vimentin, N-Cadherin, TWIST, ZEB1 ZEB2. In addition, determined Nevertheless, co-treatment did show additive induces transcription reprogramming angiogenesis, invasion, migration, proliferation. context inflammation obesity, speculate overexpression provides additional accelerating